Biotin-labeled Nucleotides are used for non-fluorescent labeling of nucleic acids in purification and detection applications. Biotin-11-dCTP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11-atom linker attached to the C5 position of cytidine. Note: The optimal final concentration of Biotin-11-dCTP may very depending on the application and assay conditions. For optimal product yields and high incorporation rates an individual optimization of the Biotin-11-dCTP/dCTP ratio is recommended. Applications: FISH[1, 2] SNP-analysis[1] Nick-translation[2] TUNEL[3] Recommended Biotin-11-dCTP/dCTP ratio for PCR and Nick Translation: 50% Biotin-11-dCTP/ 50% dCTP Synonym: gamma-[N-(Biotin-6-amino-hexanoyl)]-5-propargylamino-2-deoxy-cytidine-5-triphosphate, Triethylammonium Salt CAS Number: 136632-30-9 Molecular Formula: C28H44N7O16P3S Molecular Weight: 859.67 Appearance: Supplied as a liquid in 10mM Tris-HCl, pH 7.5. Labeled with Biotin. Purity (HPLC): 95% Concentration: ~5mM Spectroscopic Properties: lambdamax 294 nm, epsilon 9,300 M-1cm-1, Tris-HCl, pH 7.5 Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. Toxicity and Hazards: All products should be handled by qualified personnel only, trained in laboratory procedures.
Molekulargewicht:
859.67
Reinheit:
95% (HPLC)
Formulierung:
Supplied as a liquid in 10mM Tris-HCl, pH 7.5. Labeled with Biotin.
CAS Nummer:
[136632-30-9]
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