| PCR Grade nucleotides are specially manufactured and purified to the highest possible chemical purity. PCR Grade nucleotides contain at least 99% of the relevant deoxynucleoside-triphosphate (dNTP) and less than 0.9% deoxynucleoside-diphosphate (dNDP). Thymidine 5'-triphosphate sodium salt solution is used in DNA synthesis reactions such as PCR, DNA sequencing, cDNA synthesis and molecular cloning techniques. dTTP, Molecular Biology Grade, is a 100mM clear, colorless solution of the sodium salt (pH 8.3). Synonyms: Thymidine 5-(Tetrahydrogen Triphosphate) Sodium Salt, Thymidine-5-Triphosphate, Sodium Salt, dTTP, 2-Deoxythymidine 5-triphosphate trisodium salt Usage: 250ul at 100mM = 6,250 standard reactions 1,250ul at 100mM = 31,250 standard reactions CAS No: 18423-43-3 Free Acid: 365-08-2 Purity: 99% (HPLC) Appearance: Supplied as a liquid dNDP: 0.9% pH: 8.3 Quality Control: Functionally tested with Sequenase, Taq DNA Polymerase, T7 DNA Polymerase and Pfu DNA Polymerase. Concentration: 100mM Lambda Max: 2672nm A250/A260: 0.65 0.03 A280/A260: 0.73 0.03 Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. (TM)The Polymerase Chain Reaction (PCR) process is covered by patents owned by Hoffman-La Roche. |
