Disulfide Oxidoreductase, Recombinant (dsbA)

Produktübersicht

• Artikelname: Disulfide Oxidoreductase, Recombinant (dsbA)
• Artikelnummer: USB-D3778
• Hersteller Artikelnummer: D3778
• Alternativnummer: USB-D3778-50
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

DsbA appears to be necessary for correct formulation of disulfide bonds in exported proteins in vivo. DsbA is useful as a standard in immunoblotting. This protein catalyses the reduction and exchange of disulfide bonds and the oxidation of free sulfhydryl groups in vitro. It is the strongest oxidant of the thioredoxin superfamily. This thio/disulfide oxidoreductase is required for efficient disulfide bond formation in the periplasm of E. coli. DsbA is a periplasmic protein isolated from E. coli having a molecular mass of 23.15kD. Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ly-Lys-Ala-Trp. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Molekulargewicht: 23.15
• UniProt: P0AEG4
• Reinheit: 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
• Formulierung: Supplied as a lyophilized powder in 50mM sodium phosphate, 100mM sodium chloride. Reconstitute with sterile ddH2O to 0.1mg/ml.