DNA Marker 50bp Ladder, 13 Fragments (50-1000bp), BioGenomics(TM)

Produktübersicht

• Artikelname: DNA Marker 50bp Ladder, 13 Fragments (50-1000bp), BioGenomics(TM)
• Artikelnummer: USB-D3930-05
• Hersteller Artikelnummer: D3930-05
• Alternativnummer: USB-D3930-05-50,USB-D3930-05-5X50
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

DNA Marker 50bp Ladder is designed for sizing and approximate quantification of wide range double-stranded DNA on agarose and polyacrylamide gels. The ladder is composed of 13 chromato- graphically purified individual DNA fragments (in base pairs): 1000, 900, 800, 700, 600, 500, 400, 300, 250, 200, 150, 100, 50. Note: The bright bands at 500 and 250bp serve as the reference. Concentration: 0.5mg DNA/ml Storage Buffer: 10mM Tris-HCl (pH 7.6), 1mM EDTA 6X Loading Dye Solution: Supplied as a liquid in 10mM Tris-HCl, pH 7.6, 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol, 60mM EDTA. Quality Control: Analysis of 0.5ug (1ul) of the DNA Ladder on agarose gel and 1ug (2ul)-on polyacrylamide gel by ethidium bromide staining generates 13 discrete band patterns. Quantity of DNA in Band: Fragment (bp) % ng/0.5ug DNA 1000 6 30 900 6 30 800 6 30 700 6 30 600 6 30 500 15 75 400 6 30 300 6 30 250 15 75 200 7 35 150 7 35 100 7 35 50 7 35 Total 100 500 Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.