| In cryobiology DMSO has been used as a cryoprotectant and is still an important constituent of cryoprotectant vitrification mixtures used to preserve organs, tissues, and cell suspensions. Without it, up to 90% of frozen cells will become inactive. It is particularly important in the freezing and long-term storage of embryonic stem cells and hematopoietic stem cells, which are often frozen in a mixture of 10% DMSO, a freezing medium, and 30% fetal bovine serum. In the cryogenic freezing of heteroploid cell lines (MDCK, VERO, etc.) a mixture of 10% DMSO with 90% EMEM (70% EMEM + 30% fetal bovine serum + antibiotic mixture) is used. Synonyms: Methyl Sulfoxide, Dimethylsulfoxide, DMSO CAS No: 67-68-5 Molecular Formula: C2H6SO Molecular Weight: 78.13 Purity: 99% Appearance: Colorless, clear liquid at RT Specific Gravity: 1.095-1.097 Boiling Point: 188-190C Congealing Temp: 18.3C Refractive Index: 1.4755-1.4775 pKa: 31.3 Water (KF): 0.1% Titratable Acid: 5ml Residue after Evaporation: 0.01% Solubility: Water (very soluble) RNase: None Detected DNase: None Detected Storage and Stability: Store at RT. Stable for 12 months at RT after receipt. Note: DMSO will congeal easily and may remain a solid at cool temperatures. Warm slightly to return to liquid state. |
