| Control peptide for D8055-01 (affinity purified) and D8055-01A (antiserum). The peripheral nerve regeneration entails sequential changes in the expression of thousands of genes. These changes are necessary to protect the damaged neurons from death, activate the surrounding glial cells, and accelerate the neurite elongation. The nerve regeneration requires a wide range of active proteases including few members of the M-13 metalloprotease family. The M-13 family comprises several zinc-dependent metalloproteases like (DINE), PHEX, KELL, ECE, XCE, neprilysin (NEP) and neprilysin-like proteases (NEPLs). The NEPLs (NEPL-alpha, NEPL-alpha, NEPL-gamma) arise from the alternative splicing of a single NEPL gene and are zinc dependent metalloproteases with ~54% homology to NEP. DINE (damage-induced neuronal endopeptidase) or Endothelin converting enzyme-like 1 (ECEL1) or X-converting enzyme (Xce) is a 95kD (mouse/rat/human 775aa, chromosome 2q36-q37), type II integral membrane metalloprotease containing a conserved zinc-binding motif and an ENXADX consensus sequence, consistent with gluzinitin. The aa sequence of DINE is 36 and 32% identical to ECE-1 and NEP, respectively. But, DINE is devoid of enzyme activity like ECE. Unlike NEP, DINE has no proteolytic activity to A beta. However, the enzyme can hydrolyze synthetic NEP substrates and thiorphan, EDTA and phosphoramidon inhibit its activity. DINE also inhibits C2-ceramide induced apoptosis in COS-7 cells. Although, the endogenous substrate for DINE is yet to be identified, its proteolytic activity activates, at least in part, free radical scavenging in damaged neurons. The DINE expression is restricted to brain tissue with predominant expression in hypothalamus, large cholinergic cells in striatum and low expression in virtually all regions of brain except in cerebral cortex, hippocampus and cerebellum. DINE, expression is markedly increased in response to optic, spinal sensory, cortical and thalamic nerve injury. Source: Synthetic peptide corresponding to 17aa sequence mapping nears the cytoplasmic N-terminus of rat DINE. Molecular Weight: ~3kD Applications: Suitable for use in ELISA and Antibody Blocking. Not suitable for Western Blot. Other applications not tested. Recommended Dilution: ELISA: 5-10ug per 1ul of antiserum or 1ug of aff pure IgG. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
