Dipeptidyl Peptidase 9, BioAssay(TM) Kit (DPP9)

Produktübersicht

• Artikelname: Dipeptidyl Peptidase 9, BioAssay(TM) Kit (DPP9)
• Artikelnummer: USB-D8065-31Q
• Hersteller Artikelnummer: D8065-31Q
• Alternativnummer: USB-D8065-31Q-96
• Hersteller: US Biological
• Kategorie: Kits/Assays

Produktbeschreibung

Dipeptidyl peptidase-9 (DPP9) is a serine exopeptidase that cleaves Xproline dipeptides from the N-terminus of polypeptides. DPP9 exhibits similar activity to that of DPP4 but unlike DPP4, DPP9 is not membrane bound. DPP9 activity has been linked to several diseases, including type 2 diabetes, obesity and cancer. The DPP9 Fluorescent Activity Assay kit is designed to measure DPP9 activity using purified DPP9 or cell extracts containing DPP9. It comes in a convenient 96-well format, with purified DPP9 enzyme, DPP9 substrate, and DPP9 assay buffer for 100 enzyme reactions. The key to the DPP9 Fluorescent Activity Assay is the fluorogenic substrate. Using this kit, only one simple step on a microtiter plate is required for DPP9 reactions. The DPP9 fluorometric substrate is incubated with a sample containing DPP9 enzyme to produce a fluorophore that can then be measured using a fluorescence reader. APPLICATIONS: Suitable for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications. Other applications not tested. Kit Components: *D8065-31Q1: DPP9 Enzyme Recombinant Human, 1x5ug D8065-31Q2: DPP Assay Buffer: 1x10ml *D8065-31Q3: Fluorogenic DPP Substrate 1, 0.5mM in DMSO, 1x100ul D8065-31Q4: AMC Fluorescent standard (50uM), 1x500ul D8065-31Q5: Microtiter Plate, 1x96wells Storage and Stability: Store *D8065-31Q1 and *D8065-31Q3 at -70C. Store D8065-31Q5 at RT. Store other components at -20C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.