Control Peptide for D9016-43 (antiserum) and D9016-44 (affinity purified). Dopamine is an endogenous catecholamine that influences many cellular activities, including behavior, hormone synthesis and release, blood pressure and intracellular ion transport. A family of at least 5 Dopamine Receptors (DR) genes, D1-D5, have been identified based upon the amino acid identity, pharmacological specificity and effector responses. DR have been classified into either the D1-like (D1, D1B, and D5) or D2-like (D2, D3, and D4). Dopamine D2 receptor is known to exist in the short (D2S) and the long (D2L) forms that are encoded by splice variants of a single gene and differ only by the presence of an additional 29aa in the intracellular, cytoplasmic loop 3 (1). D2L receptor is a 446aa, G-protein coupled, and transmembrane receptor protein. D2R may play a role in the coupling of the receptor to G-proteins. All members of this family have similar structure and contain 7 putative transmembrane domains. Source: A 28aa synthetic peptide sequence from the human D2 Receptor, within the cytoplasmic loop 3, coupled to KLH. The 28aa sequence is shared by both the long and short form. Sequence Homology: Human, bovine, canine-100%, Xenopus-50% with D2 receptors. No significant sequence homology is seen with other dopamine receptors (D1 and D3-D5). Applications: Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot. Other applications not tested. Recommended Dilution: ELISA: Coat plates with 1ug/ml. Antibody Blocking: Use 5-10ug per 1ug of affinity purified antibody (D9016-44) or 1ul of antiserum (D9016-45). Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Reinheit:
Highly purified
Formulierung:
Supplied as a liquid in PBS, pH 7.5, 0.05% sodium azide.
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