Biotin-labeled Nucleotides are used for non-fluorescent labeling of nucleic acids in purification and detection applications. Applications: Suitable for use in SNP Analsis, In situ hybridization detection, Excision repair patches, Southern Blot and DNA-labeling. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Spectroscopic Properties: lambda max 240/289nm, epsilon 10.7/7.1 L mmol-1 cm-1 (Tris-HCl, pH 7.5) Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molekulargewicht:
749.51
Reinheit:
~95%
Formulierung:
Supplied as a liquid in 10mM Tris-HCl, pH 7.5. Labeled with Biotin.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten