Eco24I (HgiJII)

Produktübersicht

• Artikelname: Eco24I (HgiJII)
• Artikelnummer: USB-E0373-50
• Hersteller Artikelnummer: E0373-50
• Alternativnummer: USB-E0373-50-1500
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

5-G Pu G C Py C-3 3-C Py C G Pu G-5 Concentration: 10u/ul Source: E scherichia coli RFL24 Buffer: 33mM Tris-acetate (pH 7.9), 10mM magnesium acetate, 66mM potassium acetate and 0.1mg/ml BSA. Incubate at 37C Diluent Buffer: 10mM Tris-HCl (pH 7.4 at 25C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. For longer periods-the Storage Buffer should be used. Storage Buffer: 10mM Tris-HCl (pH 7.5 at 25C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol. Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with Eco24I. Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with Eco24I, more than 95% of the DNA fragments can be ligated at a 5-termini concentration of 0.07uM. More than 95% of these can be recut. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours. Blue/White Cloning Assay: pUC19 DNA was digested at a unique site with 10 units of enzyme for 16 hours. After religation and transformation 0.1% of white colonies were detected. Stability during Prolonged Incubation: A minimum of 0.2 units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37C. Thermal Inactivation: Enzyme is inactivated by incubation at 65C for 20min. Digestion of Agarose-embedded DNA: A minimum of 5 units of enzyme is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours. Compatible Ends: GGGCC C-ApaI, BseSI, SduI GAGCT C-Alw21I, SacI, SduI