Eco32I (EcoRV)

Produktübersicht

• Artikelname: Eco32I (EcoRV)
• Artikelnummer: USB-E0374
• Hersteller Artikelnummer: E0374
• Alternativnummer: USB-E0374-2000,USB-E0374-5X2000
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

5-G A T A T C-3 3-C T A T A G-5 Concentration: 10u/ul, 50u/ul Source: Escherichia coli RFL32 Buffer: 10mM Tris-HCl (pH 8.5), 10mM MgCl2, 100mM KCl and 0.1mg/ml BSA. Incubate at 37C Diluent Buffer: 10mM Tris-HCl (pH 7.4 at 25C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. For longer periods-the Storage Buffer should be used. Storage Buffer: 25mM Tris-HCl (pH 7.5 at 25C), 200mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol. Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with Eco32I. Ligation Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with Eco32I, more than 95% of the DNA fragments can be ligated at a 5-termini concentration of 0.21µM. More than 95% of these can be recut. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours. Blue/White Cloning Assay: pUC57 was digested at a unique site with 10 units of enzyme for 16 hours. After religation and transformation 0.3% of white colonies were detected. Stability during Prolonged Incubation: A minimum of 0.1 units of enzyme is required for complete digestion of 1µg of lambda DNA in 16 hours at 37C. Thermal Inactivation: Enzyme is inactivated by incubation at 65C for 20min. Digestion of Agarose-embedded DNA: A minimum of 5 units of enzyme is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours.