Eco81I (SauI)

Produktübersicht

• Artikelname: Eco81I (SauI)
• Artikelnummer: USB-E0374-40
• Hersteller Artikelnummer: E0374-40
• Alternativnummer: USB-E0374-40-500,USB-E0374-40-2500
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

5-C CT N A G G-3 3-G G A N TC C-5 Source: Escherichia coli RFL81 Concentration: 10u/ul Incubation Temperature: 37C Supplied with: R1625: Restriction Enzyme Buffer A, 10X: Dilute to 1X for use. 1X buffer composition is 33mM Tris-acetate pH 7.9, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA. Diluent Buffer: 10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol Storage Buffer: Supplied as a liquid in10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol Stability during Prolonged Incubation: A minimum of 0.1 units of Eco81I is required for complete digestion of 1ug of lambda DNA in 16 hours at 37C. Compatible Ends: BbvCl, Bpu10I, Bpu1102I, HpyF3l Thermal Inactivation: Eco81I is inactivated by incubation at 80C for 20 minutes. Number of Recognition Sites in DNA: Lambda: 2 M13mp18/19: 1 PhiX174, pBR322, pUC18/19, pUC57, pTZ19R/U: 0 Unit Definition: One unit is defined as the amount of Eco81I required to digest 1ug of lambda DNA-HindIII fragments in 1 hour at 37C in 50ul of assay buffer. Digestion of Agarose-embedded DNA: A minimum of 5 units of Eco81I is required for complete digestion of 1ug of agarose-embedded lambda DNA in 16 hours. Methylation Effects: Dam, Dcm, CpG, EcoKl: Never overlaps - No effect. EcoBl: May overlap - Effect not determined. Quality Control: Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion with Eco81I (10u/ug lambda DNA x 16 hours). Ligation/Recutting Assay: The ligation and recleavage assay was replaced with L0 test after validating experiments showed L0 test ability to trace nuclease and phosphatase activities wiht sensitivity that is higher than L/R by a factor of 100. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of Eco81I for 4 hours. Blue/White Cloning Assay: The B/W assay was replaced with L0 test after validating experiments showed L0 test ability to detect nuclease and phosphatase activities with sensitivity that equals to that of B/W test. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.