Eco105I (SnaBI)

Produktübersicht

• Artikelname: Eco105I (SnaBI)
• Artikelnummer: USB-E0374-55
• Hersteller Artikelnummer: E0374-55
• Alternativnummer: USB-E0374-55-600,USB-E0374-55-3000
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

5-T A C G T A-3 3-A T G C A T-5 Concentration: 10u/ul Buffer: 10mM Tris-HCl (pH 7.5), 10mM MgCl2 and 0.1mg/ml BSA. Incubate at 37C Diluent Buffer: 10mM Tris-HCl (pH 7.4 at 25C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol. Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 15-fold overdigestion (10u/ug lambda DNA x 1 hour) with Eco105I (see Star Activity). Ligation/Recutting Assay: After 2-fold overdigestion (2u/ug DNA x 1 hour) with Eco105I, more than 80% of the phage M13mp18 DNA fragments can be ligated at a 5-termini concentration of 0.1uM. More than 90% of these can be recut. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours. Star Activity: An excess of enzyme (20u/ug DNA x 1 hour) may result in star activity. Methylation Effects: Eco105I does not cut TAm5CGTA. Blocked by CG methylation. Stability during Prolonged Incubation: A minimum of 0.5 units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37C. Thermal Inactivation: Enzyme is inactivated by incubation at 65C for 20min. Digestion of Agarose-embedded DNA: A minimum of 5 units of enzyme is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours. Number of Recognition Sites in DNA: Lambda: 1 PhiX174: 0 M13mp18/19: 1 pBR322: 0 pUC18/19: 0 pUC57: 0 pTZ19R/U: 0 pBluescriptIIKS(-/+): 0 pBluescriptIISK(-/+): 0 pACYC177: 0 pACYC184: 0 Unit Definition: One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA-CpoI (RsrII) fragments in 1 hour at 37C in 50ul of assay buffer.