EphB3, also known as Mdk5, Hek2, or Sek4, is a 993 amino acid (aa), 110kD member of the Eph family of tyrosine kinase receptors, which engage in bidirectional signaling with members of the ephrin transmembrane ligand family. EphB3 engages ephrin B ligands. Mouse EphB3 shares 99 and 97% aa identity with rat and and human EphB3, respectively, and ~62% identity with mouse EphB1 and EphB2, within the 525 aa extracellular domain. EphB receptors are involved in central nervous system axon guidance during development or injury. EphB3 also plays multiple roles in structural organization of pre- and postnatal intestine, skin, and thymus. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile 40-50% glycerol, PBS.. Aliquot and store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
