FKBP1B, is a member of the immunophilin protein family which plays a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 (tacrolimus) and rapamycin (sirolimus). It is highly similar to the FK506-binding protein 1A. Its physiological role is thought to be in excitation-contraction coupling in cardiac muscle. Source: Recombinant corresponding to aa1-108 from human FKBP1B, fused to His-tag at N-terminal expressed in E.coli. Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH RSMGVEIETI SPGDGRTFPK KGQTCVVHYT GMLQNGKKFD SSRDRNKPFK FRIGKQEVIK GFEEGAAQMS LGQRAKLTCT PDVAYGATGH PGVIPPNATL IFDVELLNLE Molecular Weight: ~14.2kD (MALDI-TOF) Enzyme Activity: Specific activity is > 800 nmol/min/mg, and is defined as the amount of enzyme cleaves 1nmole of suc-AAFP-PNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
