FGF-10, Recombinant, Human (Fibroblast Growth Factor-10, FGFA, Keratinocyte growth factor-2)

Produktübersicht

• Artikelname: FGF-10, Recombinant, Human (Fibroblast Growth Factor-10, FGFA, Keratinocyte growth factor-2)
• Artikelnummer: USB-F4212-05A
• Hersteller Artikelnummer: F4212-05A
• Alternativnummer: USB-F4212-05A-25
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

FGF-10 is a heparin-binding growth factor that belongs to the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-10 is most related to KGF/FGF-7, and is expressed during the development and, preferentially, in adult lungs. It signals through FGFR 2b. Recombinant Human FGF-10 is a 19.3kD protein consisting of 170 amino acid residues. Recombinant corresponding to human Fibroblast Growth Factor 10 expressed in E. coli. Molecular Weight: ~19.3kD Amino Acid Sequence: MLGQDMVSPE ATNSSSSSFS SPSSAGRHVR SYNHLQGDVR WRKLFSFTKY FLKIEKNGKV SGTKKENCPY SILEITSVEI GVVAVKAINS NYYLAMNKKG KLYGSKEFNN DCKLKERIEE NGYNTYASFN WQHNGRQMYV ALNGKGAPRR GQKTRRKNTS AHFLPMVVHS Biological Activity: Assay 1: Determined by dose-dependent ability to reduce tetrazolium salt, WST-8, by dehydrogenase activities of BaF3 cells expressing FGF receptors using Cell Counting Kit-8 (CCK-8). The expected ED50 is 5ng/ml corresponding to a specific activity of 2x10e6 units/mg. Assay 2: Determined by a cell proliferation assay using 4MBr-5 cells. The expected ED50 for this effect is 0.5-15ng/ml. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile 5mM sodium phosphate, pH 7.4. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Molekulargewicht: 19.3
• UniProt: O15520
• Reinheit: 95% (SDS-PAGE, HPLC). Endotoxin: 0.1ng/ug (1EU/ug)
• Formulierung: Supplied as a lyophilized powder from PBS, pH 7.4. Reconstitute in 5mM sodium phosphate, pH 7.4 to 0.1-1mg/ml. Further dilutions should be made in aqueous buffers supplemented with carrier protein, such as 0.1-1% BSA.