Fibronectin (cold-insoluble globulin) is a high molecular weight, adhesive, glycoprotein found in both plasma and the extracellular matrix. Fibronectin is composed of two peptide chains of approximately 275,000 molecular weight which are linked through two interchain disulfide bonds at the COOH-terminal end of the molecule. The structure of fibronectin is characterized by three different types of repeating homologous sequence units. The 45 amino acid type-I repeat constitutes the NH2- and COOH-terminal ends of the protein. The two 60 amino acid type-II segments follow the first nine type-I repeats at the NH2-terminus. The 90 amino acid type-III segments occupy the central region of the fibronectin molecule. Structural differences between plasma and cellular fibronectin as well as between the two subunits of plasma fibronectin have been identified. These differences likely originate due to transcriptional and posttranscriptional events involving mRNA splicing. The apparent function of fibronectin is to mediate cell attachment by interacting with cell surface receptors and extracellular matrix components. Fibronectin contains an Arg-Gly-Asp-Ser (RGDS) cell attachment-promoting sequence within one of the type-III homology segments in the middle of the molecule. This RGDS site is recognized by specific RGDS-dependent cell receptors which are members of the integrin family of proteins. Fibronectin binding to activated platelets is mediated through the RGDS cell adhesion sequence. Localization: Plasma, 0.3mg/ml, extracellular matrix Mode of Action: Mediates cell attachment to extracellular matrix Extinction Coefficient: E1%1cm, 280nm=14.0 Structure: Heterodimer Percent Carbohydrate: 4-10%, depending on source Storage and Stability: Lyophilized powder may be stored at 4C. Stable for 12 months after receipt at 4C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at 4C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molekulargewicht:
550000
Reinheit:
95% by SDS-PAGE. Fibronectin is isolated from human plasma by thermal precipitation, ion exchange, and gelatin-agarose affinity chromatography. Purity is assessed by SDS-PAGE analysis.
Formulierung:
Supplied as a lyophilized powder from 20mM HEPES, 150mM sodium chloride, pH 7.4. Reconstiute with sterile ddH20.
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