Flk-1/KDR/vascular endothelial growth factor receptor 2, a cell membrane receptor kinase, is a high affinity receptor for vascular endothelial growth factor and is putatively involved in the growth of endothelial cells and angiogenesis. It contains seven immunoglobulin like sequences in the extracellular domain and exhibits sequence similarity to Flt-1 and Flt-4. Human VEGFR2 GenBank Accession No. AF035121), (a.a. 805-1356) with N-terminal GST tag, MW=87kD, expressed in an Baculovirus infected Sf9 cell expression system.. Stability: >6 months at -80 C Purity: >90% by SDS-PAGE. Specific Activity: 56 U/ug. One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of tyrosine substarte per minute at pH 7.4 and 30 C Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3uM Na orthovanadate, 0.5 mM ATP, 30 ug/ml Poly (Glu:Tyr)4:1 substrate, and 0.04ug recombinant VEGFR2. Application: useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
