Flt3, active, Recombinant, Human, N-terminal His-Tag Receptor Tyrosine Kinase (Fms-like Tyrosine Kinase 3, CD135)

Produktübersicht

• Artikelname: Flt3, active, Recombinant, Human, N-terminal His-Tag Receptor Tyrosine Kinase (Fms-like Tyrosine Kinase 3, CD135)
• Artikelnummer: USB-F4600-21
• Hersteller Artikelnummer: F4600-21
• Alternativnummer: USB-F4600-21-10
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

FMS-related tyrosine kinase 3 (FLT3, also called Flk2), a member of the type III receptor tyrosine kinase family that includes c-Kit, PDGF and M-CSF receptors, is expressed on early hematopoietic progenitor cells, and supports growth and differentiation within the hematopoietic system. FLT3 is activated after binding with its ligand FL, which results in a cascade of tyrosine autophosphorylation and tyrosine phosphorylation of downstream cellular targets. The p85 subunit of PI3 kinase, SHP2, GRB2 and Shc are associated with FLT3 after FL stimulation. Tyrosine 591 is located in the juxtamembrane region of FLT3. It may play an important role in regulation of FLT3 tyrosine kinase activity. Somatic mutations of FLT3 consisting of internal tandem duplications (ITDs) occur in 20% of patients with acute myeloid leukemia. Human Flt3, (aa564-end) with N-terminal His tag, expressed in an Baculovirus infected Sf9 cell expression system. GenBank Accession: NM_004119 Specific Activity: 4pmol/min/ug Assay Conditions: ADP-Glo assay was performed with 0.2mg/ml Abltide as a substrate and 100mM ATP at final concentration. Reaction was done for 45 minutes at 30C. ADP-Glo for 45 minutes at RT and Kinase detection for 55 minutes at RT. Applications: Suitable for use in the study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Produkteigenschaften

• Molekulargewicht: 55
• NCBI: 004119
• UniProt: P36888
• Reinheit: 80% by SDS-PAGE.
• Formulierung: Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 69mM sodium chloride, 1.35mM KCl, 0.025% Tween-20, 63mM Imidazole, 3mM DTT, 50% glycerol