Fractalkine, Recombinant, Human (Neurotactin, CX3CL1, FKN)
Artikelnummer:
USB-F6069-95H
Hersteller Artikelnummer:
F6069-95H
Alternativnummer:
USB-F6069-95H-20,USB-F6069-95H-50
Hersteller:
US Biological
Kategorie:
Molekularbiologie
Fractalkine is a CX3CL chemokine that signals through the CX3CR1 receptor. Fractalkine has been shown to chemoattract monocytes, microglia cells and NK cells. Fractalkine is, at this time, the only CXC3C chemokine that contains three amino acid residues between the first and second cysteine residues of the chemokine domain. The Fractalkine gene encodes for a 397 amino acid precursor protein containing a 24 amino acid signal sequence, a chemokine domain, and a mucin-like stalk sequence, which is followed by the transmembrane domain containing approximately 20 amino acids, and a C-terminal cytoplasmic domain. The extracellular chemokine domain contains 76 amino acid residues, including the four conserved cysteine residues found in other chemokines. Recombinant Human Fractalkine is an 8.5kD protein containing 76 amino acid residues, including the four conserved cysteine residues present in CC chemokines. Recombinant protein corresponding to human Fractalkine, expressed in E.coli. Amino Acid Sequence: QHHGVTKCNI TCSKMTSKIP VALLIHYQQN QASCGKRAII LETRQHRLFC ADPKEQWVKD AMQHLDRQAA ALTRNG Biological Activity: Determined by its ability to chemoattract human T cells using a concentration range of 5-10 ng/ml. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile PBS, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
