Fyn, active (Src Family Tyrosine Kinase) Recombinant, Human, His-Tag

Produktübersicht

• Artikelname: Fyn, active (Src Family Tyrosine Kinase) Recombinant, Human, His-Tag
• Artikelnummer: USB-F9500-07
• Hersteller Artikelnummer: F9500-07
• Alternativnummer: USB-F9500-07-10
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

Fyn is a member of the Src family of non-receptor protein tyrosine kinases and is is highly expressed in brain. Fyn has been shown to be involved in T cell and B cell activation and keratinocyte differentiation. Fyn can regulate the activation of PI-3 kinase in CTLL-2 cells, a murine interleukin 2 responsive cell line. In T cells, Fyn associates with the T cell antigen receptor and Thy-1. A study of human T-cells revealed that Lck and Fyn had different intracellular locations, suggesting that these two members of the Src family regulate different aspects of T cell activation. The Src homology 3 (SH3) region of Fyn can bind the proline-rich domain of the Fas ligand. In addition, Raf-1 may form a complex with Fyn through its Src Homology 2 (SH2) domain and serine phosphorylation of Raf-1. N-Terminal 6X His-Tagged, full-length human Fyn, expressed by baculovirus in Sf21 insect cells. Molecular Weight: ~61.8kD Specific Activity: 1290U/mg Unit Definition: One unit of Fyn activity is defined as 1nmol phosphate incorporated into 250uM cdc2 peptide/minute at 30C with a final ATP concentration of 100uM. Fyn Kinase Assay: ~1.7mU-1.7U phosphorylates the Src substrate peptide in vitro. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Produkteigenschaften

• Molekulargewicht: 61.8
• NCBI: 002037
• UniProt: P06241
• Reinheit: ~70% (SDS-PAGE and densitometry)
• Formulierung: Supplied as a liquid in 50mM Tris, pH 7.5, 0.15M sodium chloride, 0.1mM EGTA, 0.03% Brij-35, 0.2mM PMSF, 0.1% beta-mercaptoethanol, 1mM benzamidine, 5% glycerol.