| G418 Sulfate is an aminoglycoside related to Gentamycin, used as a selective agent in bacterial genetics. It is toxic to yeast, bacteria, many plant and mammalian cells, helminths and protozoans. Synonyms: O-2-Amino-2,7-dideoxy-D-glycero-alpha-D-gluco-heptopyranosyl-(14)-O-[3-deoxy-4-C-methyl-3-(methylamino)-beta-L-arabinopyranosyl-(16)]-2-deoxy-D-streptamine Sulfate (1:2), Antibiotic G 418 Sulfate, G418 Sulfate CAS No: 108321-42-2 Molecular Formula: C20H40N4O102H2SO4 Molecular Weight: 692.71 Appearance: White, crystalline powder Solubility: Colorless, clear, complete Potency: 620ug/mg Suggested Working Concentrations: 100-800ug/ml Mammalian Selection: 400ug/ml Maintenance: 200ug/ml Absorbance (1mg/ml): 280nm: 0.015 570nm: 0.10 Water (KF): 12% Specific Rotation: +104 to +121C Reconstitution: Reconstitute with water or ethanol to a desired stock concentration. Sterilize by filtration through a 0.2um filter, aliquot and freeze at -20C. Storage and Stability: Powder: Store at 4C. Avoid moisture uptake, as this can decrease potency. Stable for 12 months after receipt. Stock solution: Aliquot and store at -20C. Stable for 3-6 months -at 20C. |
