GABA Receptor B R2, Rat, Control Peptide (Gamma-aminobutyric Acid Receptor Type B)

Produktübersicht

• Artikelname: GABA Receptor B R2, Rat, Control Peptide (Gamma-aminobutyric Acid Receptor Type B)
• Artikelnummer: USB-G1015-50R
• Hersteller Artikelnummer: G1015-50R
• Alternativnummer: USB-G1015-50R-100
• Hersteller: US Biological
• Kategorie: Molekularbiologie
• Applikation: ELISA

Produktbeschreibung

GABA (g-amino butyric acid) is the most abundant neurotransmitter in mammalian brain. GABA exerts its effects through ionotropic ligand-gated GABAA, GABAC and GABAB receptors (GABABRs). Presynaptic GABABRs inhibit neurotransmitter release by down regulating high voltage activated, Ca+2 channels, whereas postsynaptic GABABRs decrease neuronal excitability by activating a prominent inwardly rectifying K+ (Kir) conductance that underlies the late inhibitory postsynaptic potential. Two N-terminal splice variants of GABABR1 (also called GBR1), GABABR1a (GBR1a, rat 960aa, apparent mol wt ~130kD, human 961aa) and GABABR1b (GBR1b, rat 844aa, apparent mol. wt 100kD, human 844aa) have been cloned and characterized. Human and rat GBR1a and GBR1b share 99% sequence homology. GABABRs are characterized by the presence of a cleavable signal peptide, a large extracellular N-terminus, 7 TM domains, and C-terminal, cytoplasmic domain. GABABRs are expressed in all major areas of the brain. Recently GABABR2 (or GBR2) has been cloned from rat (940aa) and human (941aa). GBR2 forms heterodimer with GBR1 through interaction at the intracellular C-terminal regions. Heterodimerization appears to be important in transporting GBR1 to the membrane and expression of functional receptors. GBR2 also colocalized with GBR1. Source: 24aa peptide of rat GBR2 Location: ~C-terminus, Cytoplasmic Species Sequence Homology: Human-100%, A 15aa and an 11aa region of the 24aa peptide is also conserved in human spliced variant 1 (182aa) and 2 (241aa). It is unknown if these splice variants are actually expressed and detectable with antibodies. No significant homology is detected with GBR1 or other receptors. Applications: ELISA: Control peptide can be used to coat ELISA plates at 1ug/ml Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Reinheit: Purified
• Formulierung: Supplied as a lyophilized powder from PBS, 0.05% sodium azide.