| Galactocerebroside (GalC) is a major galactosphingolipid of myelin which plays a role in myelination. GalC is a very useful, specific marker for oligodendroglial lineage. Applications: Suitable for use in ELISA, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: ELISA: Used with purified galactocerebrosides Immunohistochemistry: 0.5-10ug/ml on formalin-fixed frozen sections. Cannot be used on paraffin-embedded tissue sections since the antigen is denatured during embedding and paraffin removal. Immunocytochemistry: 0.5-10ug/ml on 4% paraformaldehyde, acetic acid or ethanol-fixed cultured cells. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: Immunocytochemistry: I1904-19A: IgG, F(ab)2 (FITC) Pab Gt x Mo I1904-19C: IgG, F(ab)2 (HRP) Pab Gt x Mo Western Blot: I1904-02C: IgG, H&L (HRP) Pab DkxMo I1904-03C: IgG, H&L (X-Adsorbed) (HRP) Pab DkxMo Positive Control: Neonatal cortex Storage and Stability: May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. |
