Galanthus nivalis agglutinin (GNA) is a small molecular weight tetramer consisting of subunits of about 13,000D. This lectin contains little or no carbohydrate. Unlike most mannose-specific lectins, it is not a metalloprotein and does not require Ca++ or Mn++ for binding. Binding seems to be preferentially directed toward structures containing (a-1,3) mannose residues. In contrast to most mannose-binding lectins, GNA will not bind alpha linked glucose. Reports indicate that this lectin binds IgM, but not bind IgG immunoglobulin classes of rat and mouse. The only protein from human serum reported to bind to this lectin is a2-macroglobulin. Activity (unconjugated): 15mg/ml will agglutinate human type O erythrocytes. Carbohydrate Specificity: Mannose Inhibitory Carbohydrate: Mannose a-1,3-mannose, Mannose (>0.2M ) Inhibiting/Eluting Sugar: 100mM-200mM a-methyl mannoside Storage and Stability: Stored at 4C only. Do not freeze. Stable for at least 6 months at 4C. For maximum recovery of product, centrifuge prior to removing the cap. Further dilutions can be made in assay buffer.
Molekulargewicht:
263044
Reinheit:
Purified. Three bands at 26kD, 30kD and 44kD detected by SDS-PAGE.
Formulierung:
Supplied as a liquid in PBS, pH 7.3. 0.05% sodium azide. Labeled with Agarose.
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