| Broad spectrum, cell culture antibiotic that is non toxic to viruses and mammalian cells at antibacterial levels. Inhibits growth of gram positive and negative bacteria, and mycoplasma. Inhibits normal protein synthesis by binding to L6 protein of 50S ribosomal subunit. Useful antibiotic for Baculovirus expression systems since mutations in rplF prevent Gentamycin from binding. CAS No: 1405-41-0 Molecular Formula: C21H43N5O7H2SO4 Molecular Weight: ~575.6 Appearance: Colorless, clear solution Solubility: Colorless, clear, complete Prepared from Gentamycin (Gentamicin) Sulfate: Prepared with ultrapure molecular biology grade water, 0.2um Sterile-filtered. Working Concentrations: 15-30ug/ml. Potency: 590ug Gentamycin/mg Specific Rotation: +107 to +121 Endotoxin: 0.5EU/mg Storage and Stability: May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. |
