| Broad spectrum, cell culture antibiotic that is non toxic to viruses and mammalian cells at antibacterial levels. Inhibits growth of gram positive and negative bacteria, and mycoplasma. Inhibits normal protein synthesis by binding to L6 protein of 50S ribosomal subunit. Useful antibiotic for Baculovirus expression systems since mutations in rplF prevent Gentamycin from binding. Prepared from Gentamycin (Gentamicin) Sulfate: Prepared with ultrapure molecular biology grade water, 0.2um Sterile-filtered. Potency: 590ug Gentamycin Content: C1: 25-50% C1a: 10-35% C2a+C2: 25-55% Working Concentrations: 15-30ug/ml. Identity (IR): Conforms to reference Specific Rotation: +107 to +121 Endotoxin: 10EU/ml Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
