Peptide Sequence: Gly-Ser-Ser(n-Octanoyl)-Phe-Leu-Ser-Pro-Glu-His-Gln-Arg-Val-Gln-Gln-Arg-Lys-Glu-Ser-Lys-Lys-Pro-Pro-Ala-Lys-Leu-Gln-Pro-Arg Sensitivity: Minimum Detectable Concentration: As reported Precision: Intra-assay error: <10% Inter-assay error: <15% Range: 0-100ng/ml Linear Range: As reported Plasma Extraction: Extraction is strongly recommended but not required. Specificity: Peptide %Crossreactivity Ghrelin (Human, Rat, Mouse) 100 Ghrelin 1-27 (Human) (O-n-Octanoyl-Ser3) 100 Ghrelin 1-27 (Human) (O-n-Decanoyl-Ser3) 100 Ghrelin (Human) (Des-Octanoyl-Ser3) 100 Ghrelin (Rat) (Des-Octanoyl-Ser3) 100 Ghrelin (CT) 100 Ghrelin, Gln28 (Human) 100 Ghrelin (Canine) 100 Ghrelin (Monkey) 85 Ghrelin (Porcine) 0 Secretin (Human) 0 VIP (Human, rat, mouse, porcine,sheep) 0 Prolactin-Releasing Peptide-31 (Rat) 0 Prolactin-Releasing Peptide-31 (Human) 0 Galanin (Rat) 0 Galanin (Human) 0 GH-RF (Rat) 0 GH-RF (Human) 0 NPY (Human, Rat, Mouse) 0 Orexin A (Human, Mouse, Rat, Bovine) 0 Orexin B (Human) Kit Components: G2033-72A: Assay Buffer (20x), 1x50ml G2033-72B: Microtiter Plate, 1x96 well G2033-72C: Ghrelin Pab, 1x1vial G2033-72D: Standard, 1x1 vial (1ug) G2033-72E: Peptide (Biotin), 1x1vial G2033-72F: Streptavidin (HRP), 1x30ul G2033-72G: Substrate, 1x12ml G2033-72H: Stop Solution, 2N HCI, 1x15ml G2033-72J: Positive Control, 2x200ul Storage and Stability: May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. It is recommended that the solutions be used on the same day of rehydration. Blood collection protocol and extraction procedure for plasma is provided for your information. (Materials for extraction not included).
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