| Extensively purified glucose-6-phosphate dehydrogenases have been obtained from yeasts, E. coli and various mammalian tissues, and these are generally considered to be coenzyme specific for NADP. Source: Leuconostoc mesenteroides Form: Supplied as a suspension in 3.7M ammonium sulfate. Phosphohexose isomerase, phosphogluconate dehydrogenase, adenylate kinase, and creatine phosphokinase contaminant activities 0.02%, 0.003%, 0.002%, and 0.002%, respectively. Activity: 200 NADP units per mg protein Unit Definition: 1 unit reduces 1umole of pyridine nucleotide per minute at 30C, pH 7.8 1 NAD unit = approx 1.8 NADP units AK: 0.002% CK: 0.002% 6-PGDH: 0.003% PHI: 0.02% Storage and Stability: May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. |
