Glucose Regulated Protein 94, Recombinant, Canine (Grp 94, gp96)
Artikelnummer:
USB-G3057-41
Hersteller Artikelnummer:
G3057-41
Alternativnummer:
USB-G3057-41-50,USB-G3057-41-200
Hersteller:
US Biological
Kategorie:
Molekularbiologie
Applikation:
WB
Glucose-regulated protein, Grp94 or gp96, is an abundant resident endoplasmic reticulum (ER) lumenal stress protein which together with cytosolic Hsp90 belong to the Hsp90 family of molecular chaperones. Grp94 and other resident soluble proteins of the ER such as members of the Ca(2+) binding protein subfamily (CaBP), CaBPI and CaBP2 as well as calreticulin possesses the COOH-terminal tetrapeptide Lys-Asp-Glu-Leu (KDEL) which is a sorting signal that is thought to lead to the retention of these proteins in the pre-Golgi compartments (1). Grp94 expression is upregulated by stress conditions such as glucose starvation and heat shock, which promote protein misfolding or unfolding (2). In addition to a homeostatic role in protein folding and assembly, Grp94 can function in the intracellular trafficking of peptides from the extracellular space to the MHC class I antigen processing pathway of antigen presentation cells (3,4). Grp94 and Hsp90 share high sequence identity and presumably identical adenosine nucleotide-dependent modes of regulation, but earlier data suggests that Hsp90 and Grp94 may differ in their nucleotide binding properties. The N-terminal domain of eukaryotic Hsp90 proteins contains a conserved adenosine nucleotide binding pocket which also serves as the binding site for the Hsp90 inhibitors geldanamycin and radicicol, however, the molecular basis for adenosine nucleotide-dependent regulation of Grp94 remains to be established. Recent data has identified a ligand dependent regulation of Grp94 function and suggest a model whereby Grp94 function is regulated through a ligand-dependent conversion of Grp94 from an inactive to an active conformation (5, 6). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 100ng of protein per lane should be sufficient when used in a 15-well minigel colorimetric system. The material should be diluted in an SDS containing sample buffer and heated at 100C for 5 minutes prior to use. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
