Glutamate, Vesicular Transporters 1, NT, Human, Control Peptide (VGLUT1, BNPI)

Produktübersicht

• Artikelname: Glutamate, Vesicular Transporters 1, NT, Human, Control Peptide (VGLUT1, BNPI)
• Artikelnummer: USB-G4005-61
• Hersteller Artikelnummer: G4005-61
• Alternativnummer: USB-G4005-61-100
• Hersteller: US Biological
• Kategorie: Molekularbiologie
• Applikation: ELISA, WB

Produktbeschreibung

Control Peptide for G4005-61D (antiserum) and G4005-61B (affinity purified) An 18aa synthetic peptide witin the N-terminus of human VGLUT1. Glutamate is the main excitatory neurotransmitter in the brain. To date five glutamate Transporters have been cloned: GLAST (EAAT1), GLT1 (EAAT2), EAAC1 (EAAT3), EAAT4, and EAAT5. These transporters are believed to be critical in reducing potentially toxic extracellular concentration of glutamate by rapid uptake into nerve terminals and glial cells. Most recently, vesicular type transporters for glutamate, termed VGLUTs (VGLUT1/BNPI, VGLUT2/DNPI, and EAT-4), have been cloned and characterized that are related to phosphate transporters. Although neurons exhibit Na-dependent Pi transport, the biological role of Pi uptake is not clear. Proximal tubules in the kidney reabsorb Pi in the glomeruli by the action of a group of phosphate transporters (Type 1-NaPi related, type 2-NaPi-2 related, and type 3-viral receptor-related. These receptors show weak (~20% identity) between various subtypes. A distinct type of brain specific Na+-dependent phosphate (Pi) transporter (BNPI), originally characterized as a plasma membrane transporter has been localized in a subset of glutamatergic neurons (amygdla,cereberal cortex, and hippocmaplus) and identified as VGLUT1 (rat and human 560 aa, ~60kD, ~30% homology with type-1 Pi-transporters & ~75-80% homology with VGLUT2). Interestingly, a sequence induced by subtoxic levels of NMDA in cerebelar granules also belongs to this family of transporters. BNPI/VGLUT1 is expression is restricted to the brain, where it is predominantly located in synaptic vesicles. VGLUT1 shows strong sequence homology (~48%) to EAT-4, a C. elegans protein that appears to have specific presynaptioc role in glutamtergic transmission. Applications: Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot due to low MW. Other applications not tested. Recommended Dilution: ELISA: 50-100ng per well. Antibody Blocking: 5-10ug control peptide per 1ug of G4005-61B or 1ul of G4005-61D Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Reinheit: Highly purified
• Formulierung: Supplied as a liquid in PBS, pH 7.5, 0.05% sodium azide.