Glutamic Acid Decarboxylase, Control Peptide (Glutamate Decarboxylase 65/67, GAD65/67)

Produktübersicht

• Artikelname: Glutamic Acid Decarboxylase, Control Peptide (Glutamate Decarboxylase 65/67, GAD65/67)
• Artikelnummer: USB-G7116-01A
• Hersteller Artikelnummer: G7116-01A
• Alternativnummer: USB-G7116-01A-100
• Hersteller: US Biological
• Kategorie: Molekularbiologie
• Applikation: ELISA

Produktbeschreibung

Control peptide for G7116-01. g-Aminobutyric acid (GABA) is the major known inhibitory neurotransmitter. The rate-limiting step in the synthesis of GABA is the decarboxylation of glutamate by glutamate decarboxylase (GAD, L-glutamate 1-carboxy-lyase, EC 4.1.115). In the CNS GAB is entirely restricted to GABAergic neurons. GAD is also present in the b-cells of the pancreas and autoantibodies to various GAD polypeptides are detected in insulin-dependent diabetes mellitus. Cloning of GAD genes have identified two subtypes: GAD65 (65kD, human 585aa chromosome 10) and GAD67 (67kD, human 594aa, chromosome 2) share approx. 65% aa homology. The N-terminus is the most divergent while the C-terminus is highly conserved. Although both GAD isoforms catalyzes the conversion of GABA but interact differently with the co-factor pyridoxal 5-phosphate suggesting their activities are differentially regulated. GAD67 is cytosolic, while GAD65 is membrane associated. GAD65 is a major autoantigen in diabetes mellitus and stiff-man syndrome, a rare disease of the brain. Source: Synthetic peptide corresponding to 16aa of human GAD65/67, C-terminal. Molecular Weight: ~3kD Applications: Suitable for use in ELISA and Antibody Blocking. Not suitable for Western Blot. Other applications not tested. Recommended Dilution: ELISA: 5-10ug control peptide per 1ug of aff pure IgG or 1ul antiserum Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Molekulargewicht: 3
• Reinheit: Purified
• Formulierung: Supplied as a lyophilized powder from PBS, 0.05% sodium azide.