Glutathione S-Transferase, pi, Recombinant, Human, aa1-210, His-Tag (GSTP1, GSTpi1, GST3, DFN7)

Produktübersicht

• Artikelname: Glutathione S-Transferase, pi, Recombinant, Human, aa1-210, His-Tag (GSTP1, GSTpi1, GST3, DFN7)
• Artikelnummer: USB-G8135-25D
• Hersteller Artikelnummer: G8135-25D
• Alternativnummer: USB-G8135-25D-100,USB-G8135-25D-500
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

GSTP1 is a glutathione S-transferase that belongs to the pi class. This enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur). The reactions utilizing glutathione contribute the transformation of a wide variety of electrophiles, including reactive products of lipid, protein, carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress. Recombinant protein corresponding to aa1-210 from human GSTP1, fused to His-Tag, expressed in E. coli. Swiss/Uniprot: P09211 Molecular Weight: ~27.4kD (246aa) Enzyme Activity: 80 units/mg Unit Definition: Defined as the amount of enzyme that conjugates 1 umole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione/minute at pH 6.5 at 25C. Amino Acid Sequence: MPPY TVVYFPVRGR CAALRMLLAD QGQSWKEEVV TVETWQEGSL KASCLYGQLP KFQDGDLTLY QSNTILRHLG RTLGLYGKDQ QEAALVDMVN DGVEDLRCKY ISLIYTNYEA GKDDYVKALP GQLKPFETLL SQNQGGKTFI VGDQISFADY NLLDLLLIHE VLAPGCLDAF PLLSAYVGRL SARPKLKAFL ASPEYVNLPI NGNGKQ Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Molekulargewicht: 27.4246
• NCBI: 000843
• UniProt: P09211
• Reinheit: 95% (SDS-PAGE).
• Formulierung: Supplied as a liquid in 20mM Tris-HCl, pH 7.0, 30% glycerol, 1mM EDTA, 0.1mM PMSF.