GLOD4, also known glyoxalase domain-containing protein 4, is an enzyme that belongs to the glyoxalase system. This system is a set of enzymes that carry out the detoxification of methylglyoxal and the other reactive aldehydes that are produced as a normal part of metabolism. It is a mitochondrial protein that interacts with NUDT9. Purified by using conventional chromatography. Source: Recombinant corresponding to aa1-298 of human GLOD4, fused to His-tag at N-terminal, expressed in E.coli. Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAARRALHFV FKVGNRFQTA RFYRDVLGMK VLRHEEFEEG CKAACNGPYD GKWSKTMVGF GPEDDHFVAE LTYNYGVGDY KLGNDFMGIT LASSQAVSNA RKLEWPLTEV AEGVFETEAP GGYKFYLQNR SLPQSDPVLK VTLAVSDLQK SLNYWCNLLG MKIYEKDEEK QRALLGYADN QCKLELQGVK GGVDHAAAFG RIAFSCPQKE LPDLEDLMKR ENQKILTPLV SLDTPGKATV QVVILADPDG HEICFVGDEA FRELSKMDPE GSKLLDDAMA ADKSDEWFAK HNKPKASG Molecular Weight: ~35.3kD (318aa) confirmed by MALDI-TOF Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
