Granulocyte Colony Stimulating Factor, Recombinant, Human (G-CSF)

Produktübersicht

• Artikelname: Granulocyte Colony Stimulating Factor, Recombinant, Human (G-CSF)
• Artikelnummer: USB-G8950-15
• Hersteller Artikelnummer: G8950-15
• Alternativnummer: USB-G8950-15-2,USB-G8950-15-10
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

G-CSF is a hematopoietic growth factor that stimulates the development of committed progenitor cells to neutrophils and enhances the functional activities of the mature end-cell. It is produced in response to specific stimulation by a variety of cells including macrophages, fibroblasts, endothelial cells and bone marrow stroma. G-CSF is being used clinically to facilitate hematopoietic recovery after bone marrow transplantation. Human and murine G-CSF are cross-species reactive. Recombinant human G-CSF is an 18.7 kDa protein consisting of 174 amino acid residues. Biological Activity: The ED50 as determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells is 0.1ng/ml, corresponding to a specific activity of 1x10e7 units/mg. Amino Acid Sequence: TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O and a carrier protein (0.1% BSA or HSA). Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Molekulargewicht: 18.7
• UniProt: P09919
• Reinheit: 98% by SDS-PAGE. Endotoxin: 0.1ng/ug
• Formulierung: Supplied as a lyophilized powder from 5mM sodium citrate, pH 4.0. Reconstitute with sterile ddH2O and a carrier protein to a concentration of 0.1-1mg/ml.