Human GM-CSF is a 14.6kD globular protein consisting of 128 amino acid containing two dislufied bonds. GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in by endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. Recombinant protein corresponding to aa18-144 from human GM-CSF was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. Molecular Weight: 14.6kD (128 amino acids) Amino Acid Sequence: MAPARSPSPS/ TQPWEHVNAI/ QEARRLLNLS/ RDTAAEMNET/ VEVISEMFDL/ QEPTCLQTRL/ ELYKQGLRGS/ LTKLKGPLTM/ MASHYKQHCP/ PTPETSCATQ/ IITFESFKEN/ LKDFLLVIPF/ DCWEPVQE Biological Activity: The ED50 for this effect is < 0.18 ng/ml. Measured in a cell proliferation assay using TF1 human erythroleukemic cells. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
