Green Fluorescent Protein, Recombinant (GFP)

Produktübersicht

• Artikelname: Green Fluorescent Protein, Recombinant (GFP)
• Artikelnummer: USB-G8965-10E
• Hersteller Artikelnummer: G8965-10E
• Alternativnummer: USB-G8965-10E-50,USB-G8965-10E-300
• Hersteller: US Biological
• Kategorie: Molekularbiologie
• Applikation: WB

Produktbeschreibung

GFP is a 27kD monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (395nm) and emits green light (508nm) in the absence of additional proteins, substrates, or co-factors. Intact, native GFP protein is required for fluorescence. The GFP chromophore results from the cyclization and oxidation of the sequence Ser65-Tyr66-Gly67. GFP fluorescence is stable, species independent, and suitable for a variety of applications. Full -length recombinant protein corresponding to Green Fluorescent Protein with N-terminal His-tag, expressed in E. coli. Accession: AAA27722. Applications: Suitable for use as a standard for protein gels and Western Blots (~28kD). Also suitable for calibration of uorometers and microinjection into cells and tissues. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: For long-term storage, store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Molekulargewicht: 28
• NCBI: 27722
• UniProt: P42212
• Reinheit: Purified using Ni-NTA agarose (~70%).
• Formulierung: Supplied as a liquid in PBS, 20% glycerol.