The seeds of Griffonia simlicifolia conain four distinct lectins. Two of these lectins, referred to as GS-I and GS-II have been well characterized with regard to binding specificity and structure. GS-II is specific for terminal, non-reducing alpha or beta linked N-acetyl-D-glucosamine. This lectin is not blood group specific, unlike GS-I. GS-II is also a tetramer but t is composed of identical subunits. Each subunit has a single carbohydrate binding site for GlcNAc. GS-II is the only lectin isolated that is specific for only a terminal GIcNAc residue. The subterminal saccharide does play an important role in lectin binding. Carbohydrate Specificity: Melibiose, alpha-D-Galactose Inhibitory Carbohydrate: alpha-Galactose Recommended Dilution: 20-30ug/ml is required to agglutinate fresh type B blood cells. Lectin activityy against all blood types increases after neuraminidase treatment of the cells. Optimal dilutions to be determined by the researcher. Storage and Stability May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Reinheit:
Purified
Formulierung:
Supplied as a liquid in PBS, pH 7.3, 0.5mM calcium chloride.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
