RABIF, also known as guanine nucleotide exchange factor MSS4, is a guanine-nucleotide-releasing protein that may participate in vesicular transport. This protein stimulates GTP-GDP exchange in Sec4 and Rab and binds to a subset of genetically related Rab proteins. Source: Recombinant corresponding to aa1-123 of human RABIF protein, fused to His-tag at N-terminus, expressed in E.coli. AA Sequence: MGSSHHHHHH SSGLVPRGSH MEPAEQPSELVSAEGRNRKA VLCQRCGSRV LQPGTALFSR RQLFLPSMRK KPALSDGSNP DGDLLQEHWL VEDMFIFENV GFTKDVGNIK FLVCADCEIG PIGWHCLDDK NSFYVALERV SHE Molecular Weight: 16kD (143aa), confirmed by MALDI-TOF Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
