Hemagglutinin Tag, YPYDVPDYA, HA-tag, Control Peptide (Biotin)
Artikelnummer:
USB-H1847-52L3
Hersteller Artikelnummer:
H1847-52L3
Alternativnummer:
USB-H1847-52L3-1
Hersteller:
US Biological
Kategorie:
Molekularbiologie
Applikation:
ELISA
Expression of genes in E. coli or yeast or baculovirus offers a convenient system to produce large amounts of recombinant proteins that may otherwise be difficult to isolate from natural cells and tissues. Very often antibodies to these newly identified proteins are not available to study its biochemical properties, monitor protein expression, and purification. In order to circumvent this problem, short pieces of well-defined peptides (Poly-His, Flag-epitope or c-myc epitope or HA-tag) or small proteins (bacterial GST, MBP, Thioredoxin, b-Galactosidase, VSV-Glycoprotein etc) are often cloned along with the target gene. Proteins are expressed as fusion proteins. Antibodies to these fusion-tags are already available to monitor fusion protein expression and purification. Therefore, fusion-tags serve as universal tags much like secondary antibodies. Many tags have their own characteristics. Poly-His-fusion proteins (6 x His) can bind to Nickel-Sepharose or Nickel-HRP. GST-fusion proteins can bind to glutathione-Sepharose. Therefore, a high degree of purification of fusion protein can be achieved in just one affinity purification step. Purity of fusion proteins can be followed by Tag-antibodies. Very often, fusion proteins are directly injected into animals to generate antibodies. Some fusion tags can be removed later by treatment with enzymes to generate tag-free recombinant proteins. Amino Acid Sequence: Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala Storage and Stability: Lyophilized powder may be stored at 4C for short-term only. Stable for 6 months at -20C. Reconstitute (see reconstitution instructions for peptides) and store at -20C. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molekulargewicht:
1102.27
Reinheit:
95% (HPLC)
Formulierung:
Supplied as a lyophilized powder. Labeled with Biotin.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
