Hemoglobin subunit zeta (HBZ) is belongs to the globin family. Zeta-globin is alpha-like hemoglobin. The HBZ polypeptide is synthesized in the yolk sac of the early embryo, while alpha-globin is produced throughout fetal and adult life. The HBZ gene includes five functional genes and two pseudogenes. The order of genes is: 5-zeta-pseudozeta-mu-pseudoalpha-1-alpha-2 -alpha-1-theta1-3. Recombinant human HBZ fused to His-tag at C-terminus, was expressed in E. coli. Source: Recombinant corresponding to aa1-142 of human HBZ 6x His tagged expressed in E. coli. AA Sequence: MSLTKTERTI IVSMWAKIST QADTIGTETL ERLFLSHPQT KTYFPHFDLH PGSAQLRAHG SKVVAAVGDA VKSIDDIGGA LSKLSELHAY ILRVDPVNFK LLSHCLLVTL AARFPADFTA EAHAAWDKFL SVVSSVLTEK YRLEHHHHHH Enzyme Activity: Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
