Hepatitis A Virus, VP4-VP2, Recombinant, aa5-164 (HAV)
Artikelnummer:
USB-H1900-49
Hersteller Artikelnummer:
H1900-49
Alternativnummer:
USB-H1900-49-100,USB-H1900-49-1
Hersteller:
US Biological
Kategorie:
Molekularbiologie
Applikation:
ELISA, WB
HAV, the prototype of the genus Hepatovirus, belongs to the family Picornaviridae. Its 7.5-kb single-stranded RNA genome bears different distinct regions: the 5 and 3 noncoding regions (NCR), the P1 region, which encodes the structural proteins VP1, VP2, VP3, and a putative VP4, and the P2 and P3 regions encoding nonstructural proteins associated with replication. Hepatitis A virus (HAV) encodes a single polyprotein which is posttranslationally processed into the functional structural and nonstructural proteins. Only one protease, viral protease 3C, has been implicated in the nine protein scissions. Recombinant protein corresponding to aa5-164 of HAV structural proteins VP2-VP4 immunodominant regions expressed in E. coli. Reacts strongly with human HAV positive serum. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Reinheit:
90%, Evaluated by SDS-PAGE.
Formulierung:
Supplied as a liquid in 10mM Tris-HCI, pH 9.6, 0.1% SDS and 50% glycerol.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten