| HEPES is a biological buffer (zwitterionic) designed by Good, et al., and typically referred to as Goods buffer useful in cell culture media formulations. Selection of biological buffer systems should include the following criteria: exclusion by biological membranes, low absorption between 240 and 700nm, chemically stable, and stable to temperature and concentration changes. Synonym: N-[2-Hydroxyethyl]piperazine-N-[2-ethanesulfonic acid] CAS No: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight: 238.31 Purity: 99.0% Appearance: White, crystalline powder Identity (IR): Conform to reference Solubility: Colorless, clear, complete A260nm (1M): 0.05 A280nm (1M): 0.05 A405nm (1M): 0.05 pH: 5.20.5 pKa1: ~3 pKa2: 7.550.2 Water (KF): 0.5% Residue on Ignition: 0.5% Heavy Metals: 0.001% DNase, RNase, Protease: None Detected Endotoxin: 1EU/mg |
