Hexokinase Type III, Recombinant, Human (HK3, HKIII)

Produktübersicht

• Artikelname: Hexokinase Type III, Recombinant, Human (HK3, HKIII)
• Artikelnummer: USB-H2040-50
• Hersteller Artikelnummer: H2040-50
• Alternativnummer: USB-H2040-50-100
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase3 lacks the hydrophobic N-terminal sequence critical for targeting to mitochondria. Hexpkinase3 may have anabolic functions, providing H6P for glycogen or lipid synthesis. Recombinant human Hexokinase3, fused to His tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. Molecular Weight : 101.1kD (943 amino acids) Sequences: MGSSHHHHHH/ SSGLVPRGSH/ MDSIGSSGLR/ QGEETLSCSE/ EGLPGPSDSS/ ELVQECLQQF/ KVTRAQLQQI/ QASLLGSMEQ/ ALRGQASPAP/ AVRMLPTYVG/ STPHGTEQGD/ FVVLELGATG/ ASLRVLWVTL/ TGIEGHRVEP/ RSQEFVIPQE/ VMLGAGQQLF/ DFAAHCLSEF/ LDAQPVNKQG/ LQLGFSFSFP/ CHQTGLDRST/ LISWTKGFRC/ SGVEGQDVVQ/ LLRDAIRRQG/ AYNIDVVAVV/ NDTVGTMMGC/ EPGVRPCEVG/ LVVDTGTNAC/ YMEEARHVAV/ LDEDRGRVCV/ SVEWGSFSDD/ GALGPVLTTF/ DHTLDHESLN/ PGAQRFEKMI/ GGLYLGELVR/ LVLAHLARCG/ VLFGGCTSPA/ LLSQGSILLE/ HVAEMEDPST/ GAARVHAILQ/ DLGLSPGASD/ VELVQHVCAA/ VCTRAAQLCA/ AALAAVLSCL/ QHSREQQTLQ/ VAVATGGRVC/ ERHPRFCSVL/ QGTVMLLAPE/ CDVSLIPSVD/ GGGRGVAMVT/ AVAARLAAHR/ RLLEETLAPF/ RLNHDQLAAV/ QAQMRKAMAK/ GLRGEASSLR/ MLPTFVRATP/ DGSERGDFLA/ LDLGGTNFRV/ LLVRVTTGVQ/ ITSEIYSIPE/ TVAQGSGQQL/ FDHIVDCIVD/ FQQKQGLSGQ/ SLPLGFTFSF/ PCRQLGLDQG/ ILLNWTKGFK/ ASDCEGQDVV/ SLLREAITRR/ QAVELNVVAI/ VNDTVGTMMS/ CGYEDPRCEI/ GLIVGTGTNA/ CYMEELRNVA/ GVPGDSGRMC/ INMEWGAFGD/ DGSLAMLSTR/ FDASVDQASI/ NPGKQRFEKM/ ISGMYLGEIV/ RHILLHLTSL/ GVLFRGQQIQ/ RLQTRDIFKT/ KFLSEIESDS/ LALRQVRAIL/ EDLGLPLTSD/ DALMVLEVCQ/ AVSQRAAQLC/ GAGVAAVVEK/ IRENRGLEEL/ AVSVGVDGTL/ YKLHPRFSSL/ VAATVRELAP/ RCVVTFLQSE/ DGSGKGAALV/ TAVACRLAQL/ TRV Enzyme Activity: Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Molekulargewicht: 101.1
• Reinheit: 95% by SDS PAGE
• Formulierung: Supplied as a liquid in 20mM Tris-HCl, pH8.0, 10% glycerol