HIV-1 p66, Strain IIIB, Recombinant (Reverse Transcriptase, Human Immunodeficiency Virus-1)

Produktübersicht

• Artikelname: HIV-1 p66, Strain IIIB, Recombinant (Reverse Transcriptase, Human Immunodeficiency Virus-1)
• Artikelnummer: USB-H6003-15H
• Hersteller Artikelnummer: H6003-15H
• Alternativnummer: USB-H6003-15H-50
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

Full length Recombinant heterodimeric HIV-1 IIIB p66 (RT) produced in E.coli expression system. Specificity: This protein shows reactivity with murine monoclonal antibodies and human polyclonal antibodies in ELISA and Western ELISA. Biological Activity: 0.1 unit of purified RT incorporates a minimum of 5 picomoles of dTTP per minute under standard RT assay conditions. Applications: Suitable for use in Dot Blot, ELISA and Western Blot. Other applications not tested. Recommended Dilutions: A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western Blot require 1-10ng protein depending on the nature and affinity of the test antibody. Solid phase antigen in 50-100ng amounts shows reactivity with human serum antibodies in Dot Blot assays. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing. Aliquots are stable 6 months after receipt.at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Produkteigenschaften

• Reinheit: Purified by ion-affinity chromatography to 90% purity as determined by SDS-PAGE, reduced.
• Formulierung: Supplied as a liquid in 50mM Tris, pH 8.0, 10% glycerol, 1mM DTT, 0.2M sodium chloride. No stabilizers or preservatives added.