Full length Recombinant HIV-1 (LAV) nef produced as a GST fusion protein in E. coli. Specificity: This protein shows reactivity with murine monoclonal antibodies and human polyclonal antibodies in ELISA and Western Blot. Applications: A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western Blot require 1-10ng protein depending on the nature and affinity of the test antibody. Storage and Stability: Aliquot to avoid repeated freezing and thawing and freeze at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.
Reinheit:
Purified by glutathione affinity chromatography and the purified protein is thrombin-cleaved to produce nef of 95% purity, as determined by SDS-PAGE, reduced.
Formulierung:
Supplied as a liquid in 50mM Tris, pH 8.0, 150mM sodium chloride. No stabilizers or preservatives added.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
