Coupling Ratio: ~5-10 moles of biotin per mole nef. Biological Activity: Binds to solid phase murine anti-nef monoclonal antibodies as determined by capture ELISA using strepavidin-HRP as a secondary reagent. Applications: Suitable for use in T-Cell Research, ELISA and Western ELISA. Other applications not tested. Recommended Dilutions: ELISA: 1:1000 Capture ELISA: 1-10ng Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Reinheit:
Purified by glutathione affinity chromatography and the purified protein is thrombin-cleaved to produce nef of 95% purity, as determined by SDS-PAGE, reduced.
Formulierung:
Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.09% sodium azide. Labeled with Biotin.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
