Hypoxia Inducible Factor 1 alpha (Hypoxia Inducible Factor 1 alpha Subunit Basic Helix-loop-helix Transcription Factor, HIF1A, HIF-1 alpha, HIF-1A, HIF1-alpha, ARNT Interacting Protein, Basic Helix-loop-helix PAS Protein MOP1, Mem
Artikelnummer:
USB-H9804-04H
Hersteller Artikelnummer:
H9804-04H
Alternativnummer:
USB-H9804-04H-100
Hersteller:
US Biological
Wirt:
Rabbit
Kategorie:
Antikörper
Applikation:
IHC, WB
Immunogen:
A fusion protein including aa530-825 of the mouse HIF-1 alpha protein.
Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF hydroxylases provide insight into hypoxic cell responses, which may be used to help isolate therapeutic targets. Applications: Suitable for Western Blot and Immunhistochemistry. Not recommended for Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1:500-1:2000 Immunohistochemistry (Paraffin): 5-10ug/ml Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
