Apoptosis or programmed cell death is a fundamental cellular process that is essential for normal tissue development and abnormal growth such as cancer, neurodegeneration, autoimmune diseases, and angiogenesis, etc. Apoptosis is driven by two classes of specialized proteases known as caspases (Cysteine Aspartase). The initiator caspases can be activated by self-cleavage. The effector caspases are then activated in an amplification cascade. Caspase-1 (interleukin-1 converting enzyme (ICE)) is a cytoplasmic protease that converts 34kD inactive precursor IL-1 to the mature 17kD proinflammatory cytokine. Caspase-1 is synthesized as a single-chain polypeptide zymogen consisting of an N-terminal prodomain, and a large (p20) and a small (p10) catalytic domains. A serine/threonine kinase RIP2/CARDIAK/RICK binds caspase-1 and promotes its processing. RIP2 engages caspase-1 through a direct protein-protein interaction involving corresponding caspase recruitment domains (CARDs) present at the C terminus of RIP2 and within the prodomain of caspase-1. It is now shown that activation of caspase-1 is regulated by a small CARD-containing decoy molecule termed ICEBERG. This decoy protein binds the corresponding CARD motif of caspase-1, inhibiting and/or displacing the upstream activator RIP2. Structurally, ICEBERG has resemblance to the death-domain-fold superfamily. Human Iceberg is a 90-aa protein (11q21-q22). It is 52% identical to the caspase-1 CARD. It is primarily expressed in the heart and placenta. The related CARD-containing molecules caspase-1 and RIP2 are also expressed in the heart and placenta as well as in numerous other tissues. Applications: Suitable for use in ELISA, Western Blotting. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure antibody using ECL. The predicted size of iceberg is ~ 10kD. ELISA: Control peptide can be used to coat ELISA plates at 1ug/ml and detected with antibodies (1:10-50,000 for neat serum and 0.5-1ug/ml for affinity pure). Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Reinheit:
Purified by immunoaffinity chromatography.
Formulierung:
Supplied as a liquid in PBS, pH 7.4 and 0.09% sodium azide, 40% glycerol.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
