Synthetic peptide corresponding to C-KHFRFRDLEEDP, from the internal region of human IDS (NP_000193.1)
Iduronate-2-sulfatase is required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations in this X-chromosome gene that result in enzymatic deficiency lead to the sex-linked Mucopolysaccharidosis Type II, also known as Hunter Syndrome. Iduronate-2-sulfatase has a strong sequence homology with human arylsulfatases A, B, and C, and human glucosamine-6-sulfatase. A splice variant of this gene has been described. Applications: Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 1:16,000 Western Blot: 0.1-0.3ug/ml detects a band at ~60kD in human liver lysates. Immunohistochemistry: 3-5ug/ml shows lysosomal staining of trophoblasts in paraffin embedded human placenta Optimal dilutions to be determined by the researcher. Positive Control: Human Liver lysates Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
